Application
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000Application
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | NHP2-like protein 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NHP2-like protein 1 (NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae))Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human NHP2-like protein 1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (92%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 15.5K antibody FA-1 antibody FA1 antibody NHPX antibody OTK27 antibody SNRNP15-5 antibody SNU13 antibody SPAG12 antibody SSFA1 antibody NHP2L1 antibody [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein antibody sperm specific antigen 1 antibody |
|---|---|
| Target | NHP2-like protein 1 |
| Molecular Weight(MW) | 14 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleolus |
Application
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000
Application
NHP2L1 antibody detects NHP2L1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
