Application
Immunofluorescence staining of methanol-fixed Hela cells using Niban-like protein antibody .Application
Western blot analysis of extracts from 3T3 and DU145 cells using Niban-like protein(Ab-712) antibody| Product Name | Niban-like protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Niban-like proteina maternal imprinting gene. May possess growth suppressor activity. Two major alternatively spliced isoforms have been described. forms. One form, Meg3-proximal (Meg3p), contains exons 1-3. The second form, Meg3-distal (Meg3d) did not contain exons 1-3 and was present in oocytes and in 1- and 2-cell embryos |
| Immunogen | Peptide sequence around aa.710~714 (Q-V-S-S-P) derived from Human Niban-like protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C9orf88 DKFZP434H0820 FLJ13518 FLJ22151 FLJ22298 MEG-3 MINERVA OC58 bA356B19.6 FAM129B niban-like protein 1 melanoma invasion by ERK family with sequence similarity 129 member B |
|---|---|
| Target | Niban-like protein |
| Molecular Weight(MW) | 82 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Niban-like protein antibody .
Application
Western blot analysis of extracts from 3T3 and DU145 cells using Niban-like protein(Ab-712) antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
