Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nicastrin antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 5% SDS PAGE diluted at 1:500| Product Name | Nicastrin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nicastrin (nicastrin)This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nicastrin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, BovineMouse (92%), Rat (92%), Dog (94%), Rhesus Monkey (100%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APH2 antibody KIAA0253 antibody NCSTN antibody nicastrin antibody anterior pharynx-defective 2 antibody ATAG1874 antibody |
|---|---|
| Molecular Weight(MW) | 78 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential) , Melanosome |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Nicastrin antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 5% SDS PAGE diluted at 1:500| Positive Control | 293T , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
