Nidogen antibody detects Nidogen protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Nidogen antibody diluted by 1:500
Rabbit polyclonal antibody to Nidogen (nidogen 1)This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nidogen. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, BovineMouse (88%), Bovine (87%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Secreted , extracellular space , extracellular matrix , basement membrane
Application
Application
Nidogen antibody detects Nidogen protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Nidogen antibody diluted by 1:500
Positive Control
HepG2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.