Application
Western blot analysis of NIFK in 30 ugs of NIH3T3 cell lysate using at 1:1000.| Product Name | NIFK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NIFKNIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent. |
| Immunogen | Synthetic peptide corresponding to amino acids 75-90 of mouse NIFK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. GTX13913 detects NIFK in 30 μgs of NIH3T3 cell lysate. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | AI852665 C130020J04Rik NIFK |
|---|---|
| Target | NIFK |
Application
Western blot analysis of NIFK in 30 ugs of NIH3T3 cell lysate using at 1:1000.| Positive Control | NIH3T3 whole cell lysate |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. GTX13913 detects NIFK in 30 μgs of NIH3T3 cell lysate. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide, 0.2% GelatinConstituents: PBS |
