Application
NIP7 antibody detects NIP7 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NIP7 protein stained by NIP7 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
NIP7 antibody detects NIP7 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE NIP7 antibody dilution: 1:1000| Product Name | NIP7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NIP7 (NIP7, nucleolar pre-rRNA processing protein)Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NIP7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Chimpanzee, BovineMouse (97%), Rat (97%), Zebrafish (82%), Xenopus laevis (90%), Dog (95%), Pig (98%), Chicken (86%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSPC031 NIP7 nucleolar pre-rRNA processing protein CGI-37 KD93 |
|---|---|
| Target | NIP7 |
| Cellular Localization | Nucleus , nucleolus |
Application
NIP7 antibody detects NIP7 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NIP7 protein stained by NIP7 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
NIP7 antibody detects NIP7 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE NIP7 antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
