Application
Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of| Product Name | NKCC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NKCC2NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell. Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide. |
| Immunogen | AA109 to 129 of the rat sequence |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| We recommend a working dilution of 1:1000 (WB).: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Slc12a1 |
|---|---|
| Target | NKCC2 |
| Molecular Weight(MW) | 160kDa (note) |
Application
Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of| Application Notes | We recommend a working dilution of 1:1000 (WB).: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Rabbit immunoglobulin in 50% glycerol |
