Application
Immunofluorescence analysis of methanol-fixed A431, using NKIRAS2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | NKIRAS2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NKIRAS2 (NFKB inhibitor interacting Ras-like 2)Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NKIRAS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434N1526 antibody KBRAS2 antibody MGC74742 antibody kappaB-Ras2 antibody NKIRAS2 antibody NFKB inhibitor interacting Ras-like protein 2 antibody NF-kappa-B inhibitor-interacting Ras-like protein 2 antibody I-kappa-B-interacting Ras-like protein |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed A431, using NKIRAS2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
