Application
NLE1 antibody detects NLE1 protein by western blot analysis. 293T whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NLE1 antibody diluted by 1:500.| Product Name | NLE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NLE1 (notchless homolog 1 (Drosophila)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NLE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, Chimpanzee, BovineMouse (96%), Rat (96%), Xenopus laevis (81%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nle notchless homolog 1 (Drosophila) |
|---|---|
| Target | NLE1 |
| Molecular Weight(MW) | 53 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleolus |
Application
NLE1 antibody detects NLE1 protein by western blot analysis. 293T whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NLE1 antibody diluted by 1:500.| Positive Control | 293T , 293T nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
