Application
Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAbApplication
Figure 2:Immunofluorescence analysis of Hela cells using NME1 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.Application
Flow cytometric analysis of Jurkat cells using NME1 mAb (green) and negative control (purple).| Product Name | nm23-H1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4B2] to nm23-H1This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human NME1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAAD NDPKA NM23 NM23-H1 AWD NBS NDPK-A NDKA NB |
|---|---|
| Target | NME1 |
| Molecular Weight(MW) | 17 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb
Application
Figure 2:Immunofluorescence analysis of Hela cells using NME1 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.
Application
Flow cytometric analysis of Jurkat cells using NME1 mAb (green) and negative control (purple).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
