Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1 antibody at 1:100 dilution.| Product Name | nm23-H1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to nm23-H1 (non-metastatic cells 1, protein (NM23A) expressed in)This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human nm23-H1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigRat (95%), Dog (92%), Pig (95%), Rabbit (92%), Chicken (84%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (94%), Guinea pig (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AWD antibody GAAD antibody NB antibody NBS antibody NDKA antibody NDPK-A antibody NDPKA antibody NM23 antibody NM23-H1 antibody NME1 antibody metastasis inhibition factor nm23 antibody nucleoside diphosphate kinase A antibody granzyme A-activat |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using nm23-H1 antibody at 1:100 dilution.| Positive Control | 293T , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
