Application
Western Blot: NMDA NR2B Phosphospecific Antibody [ - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.| Product Name | NMDAR2B (phospho Tyr1336) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NMDA NR2B Subunit (phospho Tyr1336)NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 conjugated to KLH |
| Modification | p-Tyr1336 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | glutamate receptor ionotropic Nmethyl Daspartate2B NR2B glutamate receptor ionotropic Nmethyl Daspartate 2B NmethylDaspartate receptor subunit2B NMDAR2B GRIN2B N-methyl-D-aspartate receptor subunit 2B NmethylDaspartate receptor subunit 2B glut |
|---|---|
| Target | NMDAR2B (phospho Tyr1336) |
| Molecular Weight(MW) | 180 (note) |
Application
Western Blot: NMDA NR2B Phosphospecific Antibody [ - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
