Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NR2B antibody [C2C3], C-term diluted at 1:3000.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:3000Application
Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:1000| Product Name | NMDAR2B antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NMDAR2B (glutamate receptor, ionotropic, N-methyl D-aspartate 2B)N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NMDAR2B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, BovineDog (97%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC142178 antibody MGC142180 antibody NMDAR2B antibody NR2B antibody hNR3 antibody GRIN2B antibody glutamate receptor subunit epsilon-2 antibody N-methyl-D-aspartate receptor subunit 3 antibody glutamate [NMDA] receptor subunit epsilon-2 antibody |
|---|---|
| Molecular Weight(MW) | 166 kDa (note) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein |
Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NR2B antibody [C2C3], C-term diluted at 1:3000.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:3000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
