Application
Immunofluorescence analysis of methanol-fixed A431, using NMT2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using NMT2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000| Product Name | NMT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NMT2 (glycylpeptide N-tetradecanoyltransferase 2)N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NMT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, BovineMouse (96%), Rat (95%), Zebrafish (88%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NMT2 antibody, type II N-myristoyltransferase antibody, NMT 2 antibody, peptide N-myristoyltransferase 2 antibody, glycylpeptide N-tetradecanoyltransferase 2 antibody, myristoyl-CoA:protein N-myristoyltransferase 2 antibody, N-myristoyltransferase 2 antibo |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed A431, using NMT2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using NMT2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
