Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with nNOS antibody diluted at 1:1000.Application
nNOS antibody detects nNOS protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. nNOS antibody diluted at 1:500.Application
nNOS antibody detects nNOS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. nNOS antibody diluted at 1:500.| Product Name | nNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to nNOS (nitric oxide synthase 1)Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. NO is also responsible for endothelium-derived relaxing factor activity regulating blood pressure. In macrophages, NO mediates tumoricidal and bactericidal actions, as indicated by the fact that inhibitors of NO synthase (NOS) block these effects. Neuronal NOS and macrophage NOS (MIM 163730) are distinct isoforms (Lowenstein et al., 1992 [PubMed 1379716]). Both the neuronal and the macrophage forms are unusual among oxidative enzymes in requiring several electron donors: FAD (see MIM 610595), flavin mononucleotide (FMN), NADPH, and tetrahydrobiopterin.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human nNOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Xenopus laevis, Dog, RabbitHuman (100%), Xenopus laevis (100%), Dog (100%), Rabbit (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IHPS1 antibody N NOS antibody NC NOS antibody NOS antibody NOS1 antibody bNOS antibody nitric oxide synthase 1 antibody |
|---|---|
| Target | nNOS |
| Molecular Weight(MW) | 161 kDa (note) (4) |
| Cellular Localization | Cell membrane , sarcolemma; Peripheral membrane protein , Cell projection , dendritic spine (By similarity) |
Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with nNOS antibody diluted at 1:1000.
Application
nNOS antibody detects nNOS protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. nNOS antibody diluted at 1:500.
Application
nNOS antibody detects nNOS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. nNOS antibody diluted at 1:500.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
