Application
FACS analysis of PC-12 cells using nNOS antibody [EP1855Y] at a dilution of 1:600 (red) or an isotype control (green).Application
IP analysis was performed with rat brain lysate and nNOS antibody [EP1855Y] at a dilution of 1:150. The percipitate was detected by the same antibody.Application
WB analysis of mouse brain lysate (10 μg) using nNOS antibody [EP1855Y] at a dilution of 1:3000.| Product Name | nNOS antibody [EP1855Y] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1855Y] to nNOSNOS (nitric oxide synthase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin . Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane; at increased level of Ca2+, nNOS is translocated to the cytoplasm. In the cytoplasm, nNOS is dephosphorylated by calcineurin, which initiates NO production . Phosphorylation by PKA and PKC on nNOS leads to downregulation of NO production. |
| Immunogen | A synthetic peptide corresponding to residues around serine 1417 of human nNOS protein was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1855Y |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-FIPWB |
| IP: 1/150. ICC/IF: 1/200. IHC-Fr: 1/1000. WB: 1/1000 - 1/2000. Flow Cyt: 1/600. Is unsuitable for IHC-P. | |
| Species Reactivity | HumanMouseRatMarmoset |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | nNOS, NOS1, IHPS1, NOS, N-NOS, NC-NOS, bNOS, N NOS, NC NOS, NCNOS |
|---|---|
| Target | nNOS |
| Molecular Weight(MW) | 161 kDa (note) |
Application
FACS analysis of PC-12 cells using nNOS antibody [EP1855Y] at a dilution of 1:600 (red) or an isotype control (green).
Application
IP analysis was performed with rat brain lysate and nNOS antibody [EP1855Y] at a dilution of 1:150. The percipitate was detected by the same antibody.
Application
WB analysis of mouse brain lysate (10 μg) using nNOS antibody [EP1855Y] at a dilution of 1:3000.| Application Notes | IP: 1/150. ICC/IF: 1/200. IHC-Fr: 1/1000. WB: 1/1000 - 1/2000. Flow Cyt: 1/600. Is unsuitable for IHC-P. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
