Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NNT-1/BSF-3 antibody at 1:250 dilution.Application
NNT-1/BSF-3 antibody detects CLCF1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human CLCF1-transfected 293T cells 12 % SDS-PAGE NNT-1/BSF-3 antibody dilution: 1:5000Application
Immunofluorescence analysis of methanol-fixed A431, using NNT1 antibody at 1:200 dilution.| Product Name | NNT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NNT1 (cardiotrophin-like cytokine factor 1)CLCF1 belongs to the interleukin-6 (IL6; MIM 147620) family of cytokines, which are involved in cell signaling through phosphorylation of gp130 (IL6ST; MIM 600694). IL6 family members share similarity in gene structure and have a 4-helix bundle in their protein structure.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NNT-1/BSF-3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (96%), Rat (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BSF-3 antibody, BSF3 antibody, CISS2 antibody, CLC antibody, NNT-1 antibody, NNT1 antibody, NR6 antibody, CLCF1 antibody, neurotrophin-1/B-cell stimulating factor-3 antibody, cardiotrophin-like cytokine factor 1 antibody, novel neurotrophin-1 antibody, CRL |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NNT-1/BSF-3 antibody at 1:250 dilution.
Application
NNT-1/BSF-3 antibody detects CLCF1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human CLCF1-transfected 293T cells 12 % SDS-PAGE NNT-1/BSF-3 antibody dilution: 1:5000
Application
Immunofluorescence analysis of methanol-fixed A431, using NNT1 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
