Application
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:3000| Product Name | NO66 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NO66 (chromosome 14 open reading frame 169)Histone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NO66. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21802 antibody, MAPJD antibody, NO66 antibody, C14orf169 antibody, nucleolar protein 66 antibody, lysine-specific demethylase NO66 antibody, hsNO66 antibody, MYC-associated protein with JmjC domain antibody, chromosome 14 open reading frame 169 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:3000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
