Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000| Product Name | NOB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOB1 (NIN1/RPN12 binding protein 1 homolog (S. cerevisiae))In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (88%), Rat (89%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ART-4 antibody, MST158 antibody, NOB1P antibody, PSMD8BP1 antibody, NOB1 antibody, nin one binding protein antibody, protein ART-4 antibody, phosphorylation regulatory protein HP-10 antibody, PSMD8 binding protein 1 antibody, adenocarcinoma antigen recogni |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
