Application
Western blot analysis of NOD2 in Jurkat cell lysate with NOD2 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.Application
Immunocytochemistry of NOD2 in Jurkat cells with NOD2 antibody at 5 ug/mL.Application
Immunofluorescence of NOD2 in Jurkat cells with NOD2 antibody at 20 ug/mL.| Product Name | NOD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOD2This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq, Jul 2008] |
| Immunogen | NOD2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 4 ug/ml. IHC-P: 5 ug/ml. IF: 10 ug/ml | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NLRC2 , IBD1 , ACUG , BLAU , PSORAS1 , CLR16.3 , NOD2B , CD , CARD15 , nucleotide-binding oligomerization domain containing 2 |
|---|---|
| Target | NOD2 |
| Tissue Specificity | At least four isoforms of NOD2 are known to exist; this antibody will only detect the longest isoform. NOD2 has no cross-reaction with NOD1. |
Application
Western blot analysis of NOD2 in Jurkat cell lysate with NOD2 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Application
Immunocytochemistry of NOD2 in Jurkat cells with NOD2 antibody at 5 ug/mL.
Application
Immunofluorescence of NOD2 in Jurkat cells with NOD2 antibody at 20 ug/mL.| Application Notes | WB: 1 - 4 ug/ml. IHC-P: 5 ug/ml. IF: 10 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
