Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal antibody at 1:250 dilution.Application
Sample (20 ug of whole cell lysate) A: Human ESC 10% SDS PAGE diluted at 1:1000Application
ELISA detection of NODAL using for capture at a concentration of 5 µg/mL and for detection at a concentration of 1.5 µg/mL.Application
Nodal antibody detects NODAL protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Nodal antibody diluted at 1:500.Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal antibody diluted at 1:500.| Product Name | Nodal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nodal (nodal homolog (mouse))The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC138230 antibody, NODAL antibody, nodal homolog antibody, nodal homolog (mouse) antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal antibody at 1:250 dilution.
Application
Sample (20 ug of whole cell lysate) A: Human ESC 10% SDS PAGE diluted at 1:1000
Application
ELISA detection of NODAL using for capture at a concentration of 5 µg/mL and for detection at a concentration of 1.5 µg/mL.
Application
Nodal antibody detects NODAL protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Nodal antibody diluted at 1:500.
Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
