Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of methanol-fixed HeLa, using Noelin antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Noelin antibody at 1:100 dilution.| Product Name | Noelin antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Noelin (noelin)This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Noelin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, ChickenRat (100%), Xenopus laevis (100%), Chicken (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMY antibody, NOE1 antibody, NOELIN1 antibody, OlfA antibody, OLFM1 antibody, pancortin 1 antibody, olfactomedin-1 antibody, neuronal olfactomedin-related ER localized protein antibody, olfactomedin related ER localized protein antibody, noelin antibody, n |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Noelin antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Noelin antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
