Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NOL3 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using NOL3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000| Product Name | NOL3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOL3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARC antibody FLJ35304 antibody MYP antibody NOP antibody NOP30 antibody NOL3 antibody muscle-enriched cytoplasmic protein antibody nucleolar protein of 30 kDa antibody nucleolar protein 3 antibody nucleolar protein 3 (apoptosis repressor with CARD |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (7) |
| Cellular Localization | Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NOL3 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using NOL3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
