Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:500Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | NOL6 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOL6 (nucleolar protein family 6 (RNA-associated))The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOL6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21959 antibody MGC14896 antibody MGC14921 antibody MGC20838 antibody NRAP antibody UTP22 antibody bA311H10.1 antibody NOL6 antibody nucleolar protein 6 antibody nucleolar RNA-associated protein antibody nucleolar protein family 6 (RNA-associat |
|---|---|
| Molecular Weight(MW) | 128 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleolus |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:500
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | IMR32 , U87-MG , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
