Application
Immunoprecipitation of NOL8 protein from HeLa whole cell extracts using 5 μg of NOL8 antibody . Western blot analysis was performed using NOL8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
NOL8 antibody detects NOL8 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with NOL8 antibody diluted by 1:1000| Product Name | NOL8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOL8 (nucleolar protein 8)NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human NOL8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nucleolar protein 8 bA62C3.4 bA62C3.3 C9orf34 NOP132 |
|---|---|
| Target | NOL8 |
| Molecular Weight(MW) | 132 kDa (note) (3) |
| Cellular Localization | Nucleus , nucleolus |
Application
Immunoprecipitation of NOL8 protein from HeLa whole cell extracts using 5 μg of NOL8 antibody . Western blot analysis was performed using NOL8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
NOL8 antibody detects NOL8 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with NOL8 antibody diluted by 1:1000| Positive Control | HeLa , HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
