Application
NOLC1 antibody detects NOLC1 protein by western blot analysis. A. 30 μg H1299 whole cell lysate/extract B. 30 μg HCT116 whole cell lysate/extract 5 % SDS-PAGE NOLC1 antibody dilution: 1:5000Application
NOLC1 antibody detects NOLC1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg HL-60 whole cell lysate/extract 5 % SDS-PAGE NOLC1 antibody dilution: 1:5000Application
NOLC1 antibody detects NOLC1 protein at cytoplasm and nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOLC1 protein stained by NOLC1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | NOLC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOLC1 (nucleolar and coiled-body phosphoprotein 1)Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NOLC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nucleolar and coiled-body phosphoprotein 1 NOPP130 P130 NS5ATP13 NOPP140 |
|---|---|
| Target | NOLC1 |
| Cellular Localization | Nucleus , nucleolus , Cytoplasm |
Application
NOLC1 antibody detects NOLC1 protein by western blot analysis. A. 30 μg H1299 whole cell lysate/extract B. 30 μg HCT116 whole cell lysate/extract 5 % SDS-PAGE NOLC1 antibody dilution: 1:5000
Application
NOLC1 antibody detects NOLC1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg HL-60 whole cell lysate/extract 5 % SDS-PAGE NOLC1 antibody dilution: 1:5000
Application
NOLC1 antibody detects NOLC1 protein at cytoplasm and nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOLC1 protein stained by NOLC1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | H1299 , HCT116 , K562 , HL-60 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
