Application
NOP56 antibody detects NOP56 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOP56 antibody diluted at a dilution of 1:1000.Application
NOP56 antibody detects NOP56 protein at nucleolus and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOP56 protein stained by NOP56 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of NOP56 protein from 293T whole cell extracts using 5 μg of NOP56 antibody . Western blot analysis was performed using NOP56 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
NOP56 antibody detects NOP56 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NOP56 antibody diluted at 1:500.| Product Name | NOP56 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOP56Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus of human NOP56. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCA36 , NOP56 ribonucleoprotein , NOL5A |
|---|---|
| Target | NOP56 |
| Molecular Weight(MW) | 66 kDa (note) |
| Cellular Localization | Nucleus , nucleolus |
Application
NOP56 antibody detects NOP56 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOP56 antibody diluted at a dilution of 1:1000.
Application
NOP56 antibody detects NOP56 protein at nucleolus and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOP56 protein stained by NOP56 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of NOP56 protein from 293T whole cell extracts using 5 μg of NOP56 antibody . Western blot analysis was performed using NOP56 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
NOP56 antibody detects NOP56 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NOP56 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
