Application
Western blot of anti-NORE1 performed from 50 ug of 293T cell lysates overexpressed with NORE1. Primary antibody anti-NORE1 (dilution 1/1,000) was incubated ON at 4 degree C. Second rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.| Product Name | NORE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NORE1RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma. Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis. Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors. |
| Immunogen | Synthetic peptide: DNPQKFALFKRIHKDGQ, corresponding to amino acids 313-329 of NORE1. This sequence is located in the RA domain of NORE1 and is 100% conserved between human, rat and mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | MAXP1 MGC10823 RAP1 Binding Protein RAPL Ras Association (RalGDS/AF-6) Domain Family 5 RASSF3 RASSF5 |
|---|---|
| Target | NORE1 |
Application
Western blot of anti-NORE1 performed from 50 ug of 293T cell lysates overexpressed with NORE1. Primary antibody anti-NORE1 (dilution 1/1,000) was incubated ON at 4 degree C. Second rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
