Rabbit polyclonal to NOS predilutedNOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
Immunogen
Synthetic peptide (Mouse) (C terminal).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHCIHC-P
IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
Species Reactivity
HumanMousePigRatBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
nitric oxide synthase
Target
NOS
Tissue Specificity
This antibody reacts with iNOS, bNOS and eNOS.
Cellular Localization
Cytoplasmic
Application
Application
Human placenta stained with
Application
Human Placenta stained with anti-Nitric Oxide Synthase-Universal (uNOS) antibody
Application Notes
IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution and store at 4°C. Do not freeze.
Storage Buffer
PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide