Application
NOSIP antibody [N1C3] detects NOSIP protein at cytoplasm and nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NOSIP antibody [N1C3] diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NOSIP antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NOSIP antibody at 1:100 dilution.| Product Name | NOSIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOSIP (nitric oxide synthase interacting protein)Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOSIP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (90%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOSIP antibody, nitric oxide synthase-interacting protein antibody, eNOS interacting protein antibody, eNOS-interacting protein antibody, nitric oxide synthase interacting protein antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
NOSIP antibody [N1C3] detects NOSIP protein at cytoplasm and nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NOSIP antibody [N1C3] diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NOSIP antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NOSIP antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
