Application
Western blot analysis of extracts from NIH-3T3 cells, treated with Etoposide 25uM 60', using Notch 1 (Cleaved-Val1754) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Notch 1 (cleaved Val1754) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Notch 1 (cleaved Val1754)Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Notch 1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hN1, AOS5, TAN1, AOVD1 |
|---|---|
| Target | Notch 1 |
| Molecular Weight(MW) | 110 kDa (note) |
| Tissue Specificity | Detects endogenous levels of fragment of activated human Notch 1 resulting from cleavage adjacent to Val1754. (Val1744 in mouse) |
| Cellular Localization | Following proteolytical processing NICD is translocated to the nucleus. |
Application
Western blot analysis of extracts from NIH-3T3 cells, treated with Etoposide 25uM 60', using Notch 1 (Cleaved-Val1754) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
