Application
Western blot analysis of extracts from 293 cells, treated with TNF-a 20ng/ml 30', using Notch 2 (Cleaved-Asp1733) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Notch 2 (cleaved Asp1733) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Notch 2 (cleaved Asp1733)Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Notch 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOTC2, Hn2, Notch 2, Neurogenic locus notch homolog protein 2 precursor, Motch B, NTC2, Notch 2 (cleaved D1733), Notch 2 (cleaved Asp1733), Notch 2 cleaved D1733, Notch 2 cleaved Asp1733, Cleaved Notch 2 (D1733), Cleaved Notch 2 (Asp1733) |
|---|---|
| Target | Notch 2 |
| Molecular Weight(MW) | 100 and 130 kDa (note) |
| Tissue Specificity | Notch 2 (Cleaved-Asp1733) Antibody detects endogenous levels of fragment of activated Notch 2 resulting from cleavage adjacent to Asp1733. |
| Cellular Localization | Following proteolytical processing NICD is translocated to the nucleus. |
Application
Western blot analysis of extracts from 293 cells, treated with TNF-a 20ng/ml 30', using Notch 2 (Cleaved-Asp1733) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
