Application
Western blot analysis of extracts of various cell lines, using NOTCH1 antibody.WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Product Name | NOTCH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOTCH1This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human NOTCH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IP: 1:20 - 1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | notch 1 , hN1 , TAN1 |
|---|---|
| Target | NOTCH1 |
Application
Western blot analysis of extracts of various cell lines, using NOTCH1 antibody.
WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IP: 1:20 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
