Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Val1697) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | NOTCH2 (cleaved Val1697) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NOTCH2 (cleaved Val1697)Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Immunogen | The immunogen corresponding to a region within amino acids 1678 and 1727 of human NOTCH2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:10000 Optimal dilutions should be determined experimentally by the end user. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOTCH2, hN2, AGS2, NOTCH2 (cleaved V1697), NOTCH2 (cleaved Val1697), NOTCH2 cleaved V1697, NOTCH2 cleaved Val1697, Cleaved NOTCH2 (V1697), Cleaved NOTCH2 (Val1697) |
|---|---|
| Target | NOTCH2 |
| Molecular Weight(MW) | 83 kDa (note) |
| Tissue Specificity | NOTCH2 (Cleaved-Val1697) Antibody detects endogenous levels of fragment of activated NOTCH2 resulting from cleavage adjacent to Val1697. |
| Cellular Localization | Following proteolytical processing NICD is translocated to the nucleus. |
Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Val1697) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:10000 Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
