Application
IHC-P analysis of human kidney tissue using Notch 2 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.| Product Name | NOTCH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOTCH2Synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa).? A residue of cysteine was added to the amino terminal end to facilitat |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanDogChimpanzee |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HJCYS , AGS2 , HN2 |
|---|---|
| Target | Notch2 intra |
Application
IHC-P analysis of human kidney tissue using Notch 2 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide |
