Application
Western blot analysis of extracts from 293/COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | NOTCH4 (cleaved Val1432) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NOTCH4 (cleaved Val1432)Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOTCH4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC74442 NOTCH3 FLJ16302 INT3 NOTCH4 NOTCH4 (cleaved V1432) NOTCH4 (cleaved Val1432) NOTCH4 cleaved V1432 NOTCH4 cleaved Val1432 Cleaved NOTCH4 (V1432) Cleaved NOTCH4 (Val1432) |
|---|---|
| Target | NOTCH4 |
| Molecular Weight(MW) | 59 kDa (note) |
| Tissue Specificity | NOTCH4 (Cleaved-Val1432) Antibody detects endogenous levels of fragment of activated NOTCH4 resulting from cleavage adjacent to Val1432. |
| Cellular Localization | Following proteolytical processing NICD is translocated to the nucleus. |
Application
Western blot analysis of extracts from 293/COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
