Application
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/ml.Application
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 ug/ml.Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 ug/ml.| Product Name | NOX2 / gp91phox antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOX2/gp91phoxCytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
| Immunogen | Rabbit polyclonal NOX2/gp91phox antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2/gp91phox. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | p91-PHOX , CGD , IMD34 , GP91-PHOX , cytochrome b-245, beta polypeptide , GP91-1 , GP91PHOX , CYBB , AMCBX2 , NOX2 |
|---|---|
| Target | NOX2/gp91phox |
| Tissue Specificity | NOX2/gp91phox antibody is rat specific. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins. |
Application
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 ug/ml.
Application
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 ug/ml.
Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 ug/ml.| Application Notes | WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
