Application
NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. NOX4 antibody [N3C3] diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOX4 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: NOX4 protein stained by NOX4 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
NOX4 antibody detects NOX4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NOX4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NOX4 (NADPH oxidase 4)This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOX4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (84%), Bovine (96%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KOX antibody, KOX-1 antibody, RENOX antibody, NOX4 antibody, renal NAD(P)H-oxidase antibody, NADPH oxidase 4 antibody, kidney superoxide-producing NADPH oxidase antibody, kidney oxidase-1 antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (7) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein (Probable) , Cell junction , focal adhesion (Probable) , Nucleus (Probable) |
Application
NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. NOX4 antibody [N3C3] diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOX4 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: NOX4 protein stained by NOX4 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NOX4 antibody detects NOX4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
