Application
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 ug/ml.Application
Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.Application
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 ug/mL.| Product Name | Noxa antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NoxaApoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4). |
| Immunogen | Human Noxa Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a concentration of 0.5-2 μg/mL. For IHC: Use at a concentration of 1 μg/mL. For ICC/IF: Use at a concentration of 10 μg/mL. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PMAinduced protein1, PMAIP1, Noxa, PMA-induced protein 1, PMAinduced protein 1, APR, PMA induced protein 1 |
|---|---|
| Target | Noxa |
| Tissue Specificity | This antibody is specific for Noxa |
Application
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 ug/ml.
Application
Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Application
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 ug/mL.| Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 0.5-2 μg/mL. For IHC: Use at a concentration of 1 μg/mL. For ICC/IF: Use at a concentration of 10 μg/mL. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
