Application
NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.Application
NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Product Name | NPAS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NPAS2 (neuronal PAS domain protein 2)The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NPAS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ23138 antibody MGC71151 antibody MOP4 antibody PASD4 antibody bHLHe9 antibody NPAS2 antibody class E basic helix-loop-helix protein 9 antibody neuronal PAS domain-containing protein 2 antibody PAS domain-containing protein 4 antibody neuronal P |
|---|---|
| Target | NPAS2 |
| Molecular Weight(MW) | 92 kDa (note) |
| Cellular Localization | Nucleus |
Application
NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] diluted at 1:1000.
Application
NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
