Application
Western Blot: Niemann-Pick type C1 Like-1 Antibody [ - Detection of NPC3 in rat small intestine membrane preparations (20 ug) using Lane 1: 2 ug/ml. Lane 2: 4 ug/ml. ECL: 5 minute exposure.| Product Name | NPC1L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Niemann-Pick type C1 Like 1 (NPC1 L1)Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol. |
| Immunogen | A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 1000-1100). [UniProt# Q6T3U3] |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| This antibody can be used for Western blot where a band is seen at ~140 kDa, representing NPC1L1. Another band may be seen ~45 kDa. The specificity of this band is unknown. No other applications have been tested. Suggested working dilutions: *Western blot: 1:500 (ECL)*The investigator should determine the optimal working dilution for a specific application.: |
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| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | NPC11L1 antibody |
|---|---|
| Target | NPC1L1 |
| Tissue Specificity | This antibody reacts with rat NPC1L1 protein |
Application
Western Blot: Niemann-Pick type C1 Like-1 Antibody [ - Detection of NPC3 in rat small intestine membrane preparations (20 ug) using Lane 1: 2 ug/ml. Lane 2: 4 ug/ml. ECL: 5 minute exposure.| Application Notes | This antibody can be used for Western blot where a band is seen at ~140 kDa, representing NPC1L1. Another band may be seen ~45 kDa. The specificity of this band is unknown. No other applications have been tested. Suggested working dilutions: *Western blot: 1:500 (ECL)*The investigator should determine the optimal working dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of affinity purified antisera in Tris-citrate/phosphate [pH 7-8] containing 0.1% sodium azide. |
