Application
Immunohistochemical staining of human tissue sections using NR0B1 antibody| Product Name | NR0B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Nuclear Receptor DAX-1 (NR0B1)DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis. In the nucleus, DAX-1 binds to a retinoic acid-responsive element (RARE) and acts as a dominant-negative regulator of retinoic acid receptor-mediated transcription. DAX-1 gene transcription is regulated by Ad4BP/SF-1 (NR5A1) and is suppressed by androgens and the androgen receptor (AR/NR3C4); AR down-regulated DAX-1 gene transcription is mediated by Ad4BP/SF-1 in the presence of the ligand. Recently it has been shown that DAX-1 is required for testis development (Meeks et al. 2003). Mutations in the human DAX-1 gene lead to X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism. Recently two isoforms of DAX-1 has been identified. DAX-1 expression has been documented in human adrenal, brain, and testis. ESTs have been isolated from cancerous human bladder, germ cell, and lung, as well as normal human brain, pancreas and testis. |
| Immunogen | Synthetic peptide - KLH conjugated. Peptide sequence identity/similarity to Mouse: 45/55%. Rat: 40/55%. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AHX Ahc Ahch DAX-1 Dax1 DAX 1 |
|---|---|
| Target | NR0B1 |
Application
Immunohistochemical staining of human tissue sections using NR0B1 antibody| Application Notes | IHC-P:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 0.01% sodium azide |
