Application
NR1D1 antibody detects NR1D1 protein by western blot analysis. Non-transfected (-) and NR1D1-transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NR1D1 antibody detects NR1D1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR1D1 protein stained by NR1D1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.Application
NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NR1D1 antibody dilution: 1:500.Application
NR1D1 antibody detects NR1D1 protein at nucleus on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NR1D1 antibody dilution: 1:500.| Product Name | NR1D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NR1D1 (nuclear receptor subfamily 1, group D, member 1)Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NR1D1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Sheep, BovineRat (93%), Sheep (92%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EAR1 antibody THRA1 antibody THRAL antibody ear-1 antibody hRev antibody NR1D1 antibody nuclear receptor Rev-ErbA-alpha antibody rev-erbA-alpha antibody V-erbA-related protein 1 antibody Rev-ErbAalpha antibody nuclear receptor subfamily 1 group D |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
NR1D1 antibody detects NR1D1 protein by western blot analysis. Non-transfected (-) and NR1D1-transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR1D1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NR1D1 antibody detects NR1D1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR1D1 protein stained by NR1D1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.
Application
NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NR1D1 antibody dilution: 1:500.
Application
NR1D1 antibody detects NR1D1 protein at nucleus on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NR1D1 antibody dilution: 1:500.| Positive Control | Human NR1D1-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
