Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500 and competitor's antibody (SC-13063) diluted by 1:100.Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000| Product Name | NR1H4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NR1H4 (nuclear receptor subfamily 1, group H, member 4) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NR1H4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit, BovineMouse (93%), Rat (92%), Pig (92%), Rabbit (96%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAR antibody FXR antibody HRR-1 antibody HRR1 antibody MGC163445 antibody RIP14 antibody NR1H4 antibody retinoid X receptor-interacting protein 14 antibody farnesoid X nuclear receptor antibody farnesoid X-activated receptor antibody farnesol rec |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500 and competitor's antibody (SC-13063) diluted by 1:100.
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
