Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NR5A2 antibody at 1:400 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.| Product Name | NR5A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NR5A2 (nuclear receptor subfamily 5, group A, member 2)Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NR5A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Japanese medaka, Xenopus laevis, Pig, Chicken, Sheep, BovineRat (88%), Zebrafish (85%), Japanese medaka (88%), Xenopus laevis (91%), Pig (96%), Chicken (96%), Sheep (95%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B1F antibody B1F2 antibody CPF antibody FTF antibody FTZ-F1 antibody FTZ-F1beta antibody LRH-1 antibody LRH1 antibody hB1F antibody hB1F-2 antibody NR5A2 antibody nuclear receptor subfamily 5 group A member 2 antibody nuclear receptor NR5A2 ant |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NR5A2 antibody at 1:400 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NR5A2 antibody [N2C3] diluted at 1:500.| Positive Control | mouse liver , Aspc1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
