FC
HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody , and then analyzed by flow cytometry.IP
Immunoprecipitation (IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.IHC
Immunohistochemical staining of paraffin-embedded breast tissue using anti-NRBP1 mouse monoclonal antibody. ( Dilution 1:50)IHC
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-NRBP1mouse monoclonal antibody. ( Dilution 1:50)WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.IF
Anti-NRBP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NRBP1.IHC
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | MADM (NRBP1) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7C5] to NRBP1May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. |
| Immunogen | Protein expressed in 293T cell transfected with human NRBP1 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCIHCIPWB |
| WB:1:1000-1:2000 IHC:1:50 ICC/IF:1:100 FC:1:100 IP:2ug/500ul |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NRBP NRBP1 MUDPNP BCON3 MADM |
|---|---|
| Tissue Specificity | is specific for human NRBP1. |
| Cellular Localization | Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia. |
FC
HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody , and then analyzed by flow cytometry.
IP
Immunoprecipitation (IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
IHC
Immunohistochemical staining of paraffin-embedded breast tissue using anti-NRBP1 mouse monoclonal antibody. ( Dilution 1:50)
IHC
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-NRBP1mouse monoclonal antibody. ( Dilution 1:50)
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
IF
Anti-NRBP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NRBP1.
IHC
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NRBP1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | WB:1:1000-1:2000 IHC:1:50 ICC/IF:1:100 FC:1:100 IP:2ug/500ul |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
