Application
WB analysis of U2OS, CEM, K562 and HeLa lysates using NRF1 antibody at a dilution of 1:1,000.| Product Name | NRF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [9E6-H8-B6] to NRF1This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration,heme biosynthesis,and mitochondrial DNA transcription and replication.The protein has also been associated with the regulation of neurite outgrowth.Alternate transcriptional splice variants,which encode the same protein, have been characterized.Additional variants encoding different protein isoforms have been described but they have not been fully characterized.Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for ""nuclear factor(erythroid-derived 2)-like 1"" which has an official symbol of NFE2L1.[provided by RefSeq, Jul 2008]" |
| Immunogen | Purified recombinant NRF1 protein fragments expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 9E6-H8-B6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NRF1 nuclear respiratory factor 1 ALPHA-PAL |
|---|---|
| Target | TCF11/NRF1 |
| Molecular Weight(MW) | 72kDa (note) |
| Cellular Localization | Nucleus. |
Application
WB analysis of U2OS, CEM, K562 and HeLa lysates using NRF1 antibody at a dilution of 1:1,000.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide,50% glycerol,0.1%BSA. |
