Application
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal . Western blot analysis was performed using NRF2 antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2 antibody at 1:100 dilution.Application
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NRF2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHC-PIPWB |
| WB:1:200~1:1000 IP:1:50~1:200 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (99%), Bovine (84%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NRF2 antibody NFE2L2 antibody nuclear factor erythroid-derived 2-like 2 antibody nuclear factor erythroid derived 2 like 2 antibody NF-E2-related factor 2 antibody HEBP1 antibody nuclear factor erythroid 2-related factor 2 antibody NFE2-related fa |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (8) |
| Cellular Localization | Cytoplasm , cytosol , Nucleus |
Application
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal . Western blot analysis was performed using NRF2 antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2 antibody at 1:100 dilution.
Application
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T , C8D30 |
|---|---|
| Application Notes | WB:1:200~1:1000 IP:1:50~1:200 ICC/IF:1:50~1:250 IHC-p:1:25~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
