Application
Non-infected (–) and infected (+) BHK-21 cell extracts (30 μg) were separated by 10% SDS-PAGE. Left: the membrane was blotted with JEV NS1 antibody diluted at 1:5000; Right: the membrane was blotted with JEV NS1' antibody diluted at 1:20000.Application
JEV NS1' antibody detects JEV NS1' protein by immunofluorescent analysis. Sample: BHK-21 cells mock (left) and infected with JEV (right) were fixed in 4% paraformaldehyde. Green: JEV NS1' protein stained by JEV NS1' antibody diluted at 1:5000. Blue: DAPI staining.| Product Name | NS1' (JEV) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NS1' (JEV) (Japanese encephalitis virus (JEV virus) non-structural protein NS1') |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Japanese encephalitis virus NS1'. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Japanese Encephalitis Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | NS1' (JEV) |
|---|
Application
Non-infected (–) and infected (+) BHK-21 cell extracts (30 μg) were separated by 10% SDS-PAGE. Left: the membrane was blotted with JEV NS1 antibody diluted at 1:5000; Right: the membrane was blotted with JEV NS1' antibody diluted at 1:20000.
Application
JEV NS1' antibody detects JEV NS1' protein by immunofluorescent analysis. Sample: BHK-21 cells mock (left) and infected with JEV (right) were fixed in 4% paraformaldehyde. Green: JEV NS1' protein stained by JEV NS1' antibody diluted at 1:5000. Blue: DAPI staining.| Positive Control | JEV infected BHK21 cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
