Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in paraformaldehyde. Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by western blot analysis. A. 10 µg BHK-21 whole cell extract B. 10 µg whole cell extract of Japanese encephalitis virus infected BHK-21 cells C. 10 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells 10% SDS-PAGE NS3 (Japanese encephalitis virus) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH. Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.| Product Name | NS3 (JEV) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NS3 (Japanese encephalitis virus) (-) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Japanese Encephalitis Virus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 28 kDa (note) |
|---|---|
| Cellular Localization | Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Remains non-covalently associated to NS3 protease |
Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in paraformaldehyde. Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by western blot analysis. A. 10 µg BHK-21 whole cell extract B. 10 µg whole cell extract of Japanese encephalitis virus infected BHK-21 cells C. 10 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells 10% SDS-PAGE NS3 (Japanese encephalitis virus) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NS3 (Japanese encephalitis virus) antibody detects NS3 (Japanese encephalitis virus) protein by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH. Green: NS3 (Japanese encephalitis virus) protein stained by NS3 (Japanese encephalitis virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.| Positive Control | Japanese encephalitis virus infected BHK-21 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
